Chromatone is a multiscope for simultaneous multicolor imaging & multi-opsin stimulation in the brain or spinal cord.
The Chromatone system offers an integrated, multi-dimensional approach for simultaneous calcium imaging and optogenetic stimulation in freely behaving animals to observe, manipulate, and dissect greater mechanistic insights into physiology and behavior.
- Image at cellular resolution and/or stimulate up to four brain or spinal cord fields of view (FOV) simultaneously within a session
- Image using both Green and Red Fluorescent Proteins in an FOV, and simultaneously stimulate the same FOV with Excitatory/Inhibitory ChannelRhodopsins and Red-shifted opsins
- Examine the impact of optogenetic manipulation on free behavior and map direct, causal effects on various downstream network dynamics
Special Multiscope Applications
Advanced integrated imaging applications for your neuroscience research
Special Multiscope Applications
Water Experiments
The multiscope design places the electronics away from the animal's head facilitating various water behavioral assays.
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Lightweight
The four lightweight and flexible optical fiber bundles weigh less than 1.5 g in total, which minimizes interference with natural animal behaviors.
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Social Interactions
Multiscope imaging permits simultaneous recording from animal pairs in social interaction studies with minimal interference
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High Throughput
Multiscope imaging facilitiates highthroughput screening of up to four animals at a time for preclinical studies with free behavior
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Multiscope Data Processing
Customize your multiscope Ca2+ imaging and optogenetic data protocols with Imaging Analyser Software
- Live stream and simultaneously record and differentiate Ca2+ dynamics using GFPs and RFPs, control sophisticated optogenetic tools, illumination levels, pulse protocols, and behavior synchronization; identify active neurons and manually extract ROIs from each multiscope FOV
- Control RGB channels simultaneously and independently during imaging and stimulation in each implanted region
- Every ROI detected as a candidate neuron in each session is saved automatically, enabling multiple, longitudinal recordings of the same FOV
The Multiscope Solution
Lenses and Accessories
Achieve Ca2+ imaging results quickly with customizable GRIN lens connectors as a packaged solution for multiscope imaging. The Piccolo lens holder accessory ensures reproducible imaging success.
Device Synchronization
CHRONOSync – A synchronization and time tagging device for multiple events during a behavioral experiment. Compatible with the Quartet and Chromatone system, any behavioral, ephys system, or camera of choice.
Service and Support
Support services for Quartet and Chromatone systems include: hands-on-surgical training, neuronal trace extraction support, and device synchronization with other behavior equipment.