Chromatone is a multiscope for simultaneous multicolor imaging & multi-opsin stimulation in the brain or spinal cord.

The Chromatone system offers an integrated, multi-dimensional approach for simultaneous calcium imaging and optogenetic stimulation in freely behaving animals to observe, manipulate, and dissect greater mechanistic insights into physiology and behavior.

  • Image and/or stimulate up to four brain or spinal cord fields of view (FOV) simultaneously within a session
  • Image using both Green and Red Fluorescent Proteins in an FOV, and simultaneously stimulate the same FOV with Excitatory/Inhibitory ChannelRhodopsins and Red-shifted opsins
  • Examine the impact of optogenetic manipulation on free behavior and map direct, causal effects on various downstream network dynamics
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Chromatone System

Special Multiscope Applications

Advanced integrated imaging applications for your neuroscience research

Multiscope Data Processing

Chromatone Analyzer

Customize your multiscope Ca2+ imaging and optogenetic data protocols with Imaging Analyser Software

  • Live stream and simultaneously record and differentiate Ca2+ dynamics using GFPs and RFPs, control sophisticated optogenetic tools, illumination levels, pulse protocols, and behavior synchronization; identify active neurons and manually extract ROIs from each multiscope FOV
  • Control RGB channels simultaneously and independently during imaging and stimulation in each implanted region
  • Every ROI detected as a candidate neuron in each session is saved automatically, enabling multiple, longitudinal recordings of the same FOV
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The Multiscope Solution

Advanced customizable solutions to enable your multi-region imaging during free behavior easier.
Piccolo

Lenses and Accessories

Achieve Ca2+ imaging results quickly with customizable GRIN lens connectors as a packaged solution for multiscope imaging. The Piccolo lens holder accessory ensures reproducible imaging success.

ChronoSync

Device Synchronization

CHRONOSync – A synchronization and time tagging device for multiple events during a behavioral experiment. Compatible with the Quartet and Chromatone system, any behavioral, ephys system, or camera of choice.

Multiscope Support

Service and Support

Support services for Quartet and Chromatone systems include: hands-on-surgical training, neuronal trace extraction support, and device synchronization with other behavior equipment.

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